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CABRI:ICLC |
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Accession_number |
ICLC HTL01004
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Cell_line_name |
1301
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Brief_description |
Species: human, Caucasian; Tissue: blood; Tumor: leukemia, acute lymphoblastic T cell; Derived from: CCRF-CEM
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Description |
Species: human, Caucasian female 4 years old; Tissue: blood; Tumor: leukemia, acute lymphoblastic T cell; Derived from: CCRF-CEM
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Depositor |
DAKO S.p.A., Milan, Italy; DAKO A/S, Glostrup, Denmark
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Bibliographic_reference |
Dev Biol Stand 1979;42:193-197 [PMID: 223921
] |
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Morphology |
lymphoblastic, cells growing single and in clusters, grown in suspension
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Culture_conditions |
continuous culture; RPMI 1640 + 10% FBS + 2mM L-Glutamine; maintain cultures between 2 and 9x10^5 cells/ml; split 1:5-1:10 every 2 to 3 days; 37C, 5% CO2
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Viruses |
Search for viruses was not performed
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Properties |
The cell line has very long telomeres
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
-
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Species_validation |
Validated by HLA-DP beta: confirmed as human by PCR
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Karyology |
approx. tetraploid
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Freezing_medium |
90% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Further_bibliography |
Nucleic Acids Res. 1998;26(16):3651-6 [PMID: 9685479
]
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